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Western blot analysis of extracts of various cell lines, using USP42 antibody (16-318) at 1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 160s.
Western blot analysis of extracts of various cell lines, using USP42 antibody (16-318) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 160s.

USP42 Antibody

CATALOG NUMBER: 16-318

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 500-700 of human USP42 (NP_115548.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HT-29
Positive Control 2:
Raji
Positive Control 3:
SGC7901
Positive Control 4:
Mouse testis
Predicted Molecular Weight:
Observed: 170kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
USP42
Additional Names:
ubiquitin carboxyl-terminal hydrolase 42, deubiquitinating enzyme 42, ubiquitin specific protease 42, ubiquitin thioesterase 42, ubiquitin thiolesterase 42, ubiquitin-specific-processing protease 42
Ncbi Gene Id Number:
84132
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-318

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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