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Western blot analysis of extracts of various cell lines, using USP44 antibody (16-092) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using USP44 antibody (16-092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

USP44 Antibody

CATALOG NUMBER: 16-092

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-270 of human USP44 (NP_115523.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
Jurkat
Positive Control 2:
Raji
Positive Control 3:
HT-29
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse pancreas
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 81kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
USP44
Additional Names:
ubiquitin carboxyl-terminal hydrolase 44, deubiquitinating enzyme 44, ubiquitin specific protease 44, ubiquitin thioesterase 44, ubiquitin thiolesterase 44, ubiquitin-specific-processing protease 44
Ncbi Gene Id Number:
84101
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a protease that functions as a deubiquitinating enzyme. The encoded protein is thought to help regulate the spindle assembly checkpoint by preventing early anaphase onset. This protein specifically deubiquitinates CDC20, which stabilizes the anaphase promoting complex/cyclosome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-092

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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