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43-030 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is
43-030 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b
43-030 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin
43-030 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/vesicle staining. The nuclear stain is

USP6 Antibody

CATALOG NUMBER: 43-030

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HHIDLDVRTTLRNH
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in Human Testis, Prostate and Placenta lysates gave no specific signal but low background at antibody concentration up to 1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and U2OS cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected NOT to cross react to USP32 or to the TBC1D3 proteins.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
USP6
Additional Names:
USP6, ubiquitin specific peptidase 6 (Tre-2 oncogene), HRP1, TRE17, TRE2, Tre-2, USP6-short, OTTHUMP00000125301, deubiquitinating enzyme 6, hyperpolymorphic gene 1, proto-oncogene TRE-2, tre-2 oncogene, ubiquitin carboxyl-terminal hydrolase 6, ubiquitin specific peptidase 6-, ubiquitin specific protease 6 (Tre-2 oncogene), ubiquitin thioesterase 6, ubiquitin thiolesterase 6, ubiquitin-specific protease USP6, ubiquitin-specific-processing protease 6
Protein Accession Number:
NP_004496.2
Protein Gi Number:
109638751
Ncbi Gene Id Number:
9098

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-030

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

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