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Western blot analysis of extracts of various cell lines, using UTP14A antibody (19-960) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse brain using UTP14A antibody (19-960) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using UTP14A antibody (19-960) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

UTP14A Antibody

CATALOG NUMBER: 19-960

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UTP14A (NP_006640.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
A-549
Positive Control 3:
NCI-H460
Positive Control 4:
Mouse spleen
Positive Control 5:
Mouse thymus
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UTP14A
Additional Names:
NYCO16, SDCCAG16, Utp14, dJ537K23.3, U3 small nucleolar RNA-associated protein 14 homolog A, UTP14, U3 small nucleolar ribonucleoprotein, homolog A, UTP14A small subunit (SSU) processome component, antigen NY-CO-16, serologically defined colon cancer antigen 16
Ncbi Gene Id Number:
10813
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the uridine triphosphate 14 family. As an essential component of a large ribonucleoprotein complex bound to the U3 small nucleolar RNA, the encoded protein is involved in ribosome biogenesis and 18S rRNA synthesis. An autosomal retrotransposed copy of this X-linked gene exists on chromosome 13. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-960

List Size:
100 uL

List Price:

$419.00

Out of stock

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