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Western blot analysis of extracts of various cell lines, using VANGL2 antibody (23-210) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human kidney cancer using VANGL2 antibody (23-210) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using VANGL2 antibody (23-210) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

VANGL2 Antibody

CATALOG NUMBER: 23-210

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human VANGL2 (NP_065068.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
A-549
Positive Control 3:
Mouse brain
Positive Control 4:
Mouse spinal cord
Positive Control 5:
Mouse thymus
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
VANGL2
Additional Names:
VANGL2, vang-like 2 (van gogh, Drosophila), KIAA1215, LPP1, LTAP, MGC119403, MGC119404, STBM, homolog of Drosophila strabismus, loop-tail-associated protein, vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (D, STB1
Ncbi Gene Id Number:
57216
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a membrane protein involved in the regulation of planar cell polarity, especially in the stereociliary bundles of the cochlea. The encoded protein transmits directional signals to individual cells or groups of cells in epithelial sheets. This protein is also involved in the development of the neural plate.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-210

List Size:
100 uL

List Price:

$436.00

Datasheet
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