Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of VARP in K562 cell lysate with VARP antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

VARP Antibody

CATALOG NUMBER: 6673

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (75%)
Immunogen:
VARP antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human VARP.
The immunogen is located within amino acids 50 - 100 of VARP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
VARP antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
VARP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VARP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VARP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANKRD27
Additional Names:
VARP Antibody: VARP, PP12899, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein
Protein Accession Number:
NP_115515
Protein Gi Number:
116063534
Ncbi Gene Id Number:
84079
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VARP Antibody: The VPS9 ankyrin repeat protein (VARP) binds to the Rab21, a guanine nucleotide exchange factor that plays an essential role in endocytic trafficking. VARP localizes to early endosomes and is thought to regulate endosome dynamics. VARP also interacts with TI-VAMP/VAMP7, a vesicular SNARE that mediates an exocytic pathway that is crucial to neurite growth. Depletion of VARP by RNA interference impairs neurite growth, suggesting that VARP is a positive regulator of neurite growth.
Background Reference 1:
Zhang X, He X, Fu XY, et al. Varp is a Rab21 guanine nucleotide exchange factor and regulates endosome dynamics. J. Cell Sci. 2006; 119:1053-62.
Background Reference 2:
Burgo A, Sotirakis E, Simmler MC, et al. Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner in neurite growth. EMBO Rep. 2009; 10:1117-24.
Background Reference 3:
Alberts P, et al. Cross talk between tetanus neurotoxin-insensitive vesicle-associated membrane protein-mediated transport and L1-mediated adhesion. Mol. Biol. Cell 2003; 14:4207-20

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6673

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369