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Western blot analysis of extracts of U-251MG cells, using VARS2 antibody (16-066) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of U-251MG cells, using VARS2 antibody (16-066) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

VARS2 Antibody

CATALOG NUMBER: 16-066

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 864-1063 of human VARS2 (NP_065175.4).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
U-251MG
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
VARS2
Additional Names:
COXPD2VALRSL, VARSL, VARS2, valine--tRNA ligase, mitochondrial, valine tRNA ligase 2, mitochondrial (putative), valyl-tRNA synthetase like
Ncbi Gene Id Number:
57176
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a mitochondrial aminoacyl-tRNA synthetase, which catalyzes the attachment of valine to tRNA(Val) for mitochondrial translation. Mutations in this gene cause combined oxidative phosphorylation deficiency-20, and are also associated with early-onset mitochondrial encephalopathies. Alternative splicing of this gene results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-066

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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