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Western blot analysis of lysed extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239).
Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP (Phospho-Ser239).
Western blot analysis of lysed extracts from serum-treated 293 and PMA-treated C6 cells using VASP (Phospho-Ser239).

VASP (phospho Ser239) Antibody

CATALOG NUMBER: 79-178

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
VASP (Phospho-Ser239) antibody was raised against a peptide sequence around phosphorylation site of serine 238 (K-V-S (p) -K-Q) derived from Human VASP.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of VASP only when phosphorylated at serine 239.
Predicted Molecular Weight:
50 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
VASP
Additional Names:
NFKB3,
Protein Accession Number:
NP_003361.1
Protein Gi Number:
4507869
Ncbi Gene Id Number:
7408
Background:
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
Background Reference 1:
Wang HG, et al. (2005) euron; 45 (3): 389-403. Melichar VO, et al. (2004) Proc Natl Acad Sci U S A; 101 (47): 16671-6. Jensen BO, et al. (2004) Blood; 104 (9): 2775-82. Zhuang S, et al. (2004) J Biol Chem; 279 (11): 10397-407. Smolenski A, et al. (1998) J Biol Chem; 273 (32): 20029-35. Butt E, et al. (1994) J Biol Chem; 269 (20): 14509-17.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-178

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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