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To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hVaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Vaspin (38-184) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.

Vaspin Antibody

CATALOG NUMBER: 38-183

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hVaspin. Human Vaspin specific antibody was purified by affinity chromatography employing immobilized hVaspin matrix
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Indirect:
To detect hVaspin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Sandwich
To detect hVaspin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Vaspin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVaspin.
Western Blot:
To detect hVaspin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVaspin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Vaspin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
SERPINA12
Additional Names:
OL-64Serpin A12, OL-64, Vaspin
Protein Accession Number:
Q8IW75
Protein Gi Number:
74728144
Ncbi Gene Id Number:
145264
User Note:
Centrifuge vial prior to opening.
Background:
Vaspin (serpina12) is a adipocytokine expressed predominantly in visceral white adipose tissues. It has three beta sheets, nine alpha helices, and one central loop, which are distinctive structural features of Serpin family members. The

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-183

List Size:
0.1 mg

List Price:

$408.00

Datasheet
Shipping Info

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