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45-168 staining (1.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-168 (2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-168 staining (1.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

VAV2 Antibody

CATALOG NUMBER: 45-168

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig
Immunogen:
The immunogen for this antibody is: ETEAKYYRTLEDIEC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in 293 lysate (predicted MW of 100kDa according to NP_003362). Recommended for use at 1-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 2.5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity:
This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VAV2
Additional Names:
VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2
Protein Accession Number:
NP_001127870.1,NP_003362.2
Protein Gi Number:
40549448
Ncbi Gene Id Number:
7410
Background Reference 1:
Henske EP, Short MP, Jozwiak S, Bovey CM, Ramlakhan S, Haines JL, Kwiatkowski DJ. Identification of VAV2 on 9q34 and its exclusion as the tuberous sclerosis gene TSC1. Ann Hum Genet. 1995 Jan;59 ( Pt 1):25-37.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-168

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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