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46-568 (0.5ug/ml) staining of Human Bobe Marrow lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-568 (7.5ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
46-568 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
46-568 (0.5ug/ml) staining of Human Bobe Marrow lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VAV3 Antibody

CATALOG NUMBER: 46-568

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-SGEQGTLKLPEK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in Human Bone Marrow lysates (calculated MW of 97.8kDa according to NP_006104.4). Recommended concentration: 0.5-1.5ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 7.5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).
Positive Control 1:
Human Bone Marrow Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VAV3
Additional Names:
VAV3, vav 3 oncogene, vav 3 guanine nucleotide exchange factor, FLJ40431
Protein Accession Number:
NP_006104.4,NP_001073343.1
Protein Gi Number:
120432044
Ncbi Gene Id Number:
10451
Background Reference 1:
Movilla N, Bustelo XR. Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins. Mol Cell Biol. 1999 Nov;19(11):7870-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-568

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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