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IHC testing of FFPE human tonsil with VCAM-1 antibody (clone CDLA106).
IHC testing of FFPE human tonsil with VCAM-1 antibody (clone CDLA106).

VCAM-1 Antibody [CDLA106]

CATALOG NUMBER: 33-994

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC-P
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human protein was used as the immunogen for the VCAM-1 antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Titering of the VCAM-1 antibody may be required for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
VCAM1
Additional Names:
Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106, VCAM1, L1CAM
Ncbi Gene Id Number:
7412
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
Vascular cell adhesion molecule-1 (VCAM-1), also called L1CAM and CD106, is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-994

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

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