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Western blot analysis of lysates from Mouse spleen using VCAM-1/CD106 Rabbit mAb (A25822) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60 s.
Western blot analysis of various lysates using VCAM-1/CD106 Rabbit mAb (A25822) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Negative control (NC): RAW 264.7 | Exposure time: 90 s.
Western blot analysis of lysates from Rat spleen using VCAM-1/CD106 Rabbit mAb (A25822) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45 s.
Flow cytometry: 1X10^6 293T cells (negative control,left) and 293T (Transfection,right) cells were surface-stained with VCAM-1/CD106 Rabbit mAb (A25822,2 μg/mL,orange line) or Rabbit IgG isotype control (AC042,2 μg/mL,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042,2 μg/mL,left) or VCAM-1/CD106 Rabbit mAb (A25822,2 μg/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Flow cytometry: 1X10^6 RAW 264.7 cells (negative control,left) and MS1 cells (right) were surface-stained with VCAM-1/CD106 Rabbit mAb (A25822,2 μg/mL,orange line) or Rabbit IgG isotype control (AC042,2 μg/mL,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 MS1 cells were surface-stained with Rabbit IgG isotype control (AC042,2 μg/mL,left) or VCAM-1/CD106 Rabbit mAb (A25822,2 μg/mL,right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.
Western blot analysis of lysates from Mouse spleen using VCAM-1/CD106 Rabbit mAb (A25822) at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60 s.

VCAM-1/CD106 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-164

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, WB
Application Note:
WB,1:1000 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse spleen, NIH/3T3, Rat spleen
Predicted Molecular Weight:
38 kDa/81 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Vcam1
Additional Names:
CD106; Vcam-1
Protein Accession Number:
P29533
Ncbi Gene Id Number:
22329
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable integrin binding activity and primary amine oxidase activity. Acts upstream of or within several processes, including cellular response to glucose stimulus; chorio-allantoic fusion; and heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Located in apical plasma membrane. Is expressed in several structures, including brain; embryo mesenchyme; extraembryonic component; heart; and hemolymphoid system. Human ortholog(s) of this gene implicated in hypertension. Orthologous to human VCAM1 (vascular cell adhesion molecule 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-164

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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