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Western blot analysis of VDAC1 Antibody in HL-60, Y79 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VDAC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of VDAC1 using rabbit polyclonal VDAC1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VDAC1 gene.
Western blot analysis of VDAC1 Antibody in HL-60, Y79 cell line lysates (35ug/lane)

VDAC1 Antibody

CATALOG NUMBER: 62-367

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rabbit, Rat
Immunogen:
This VDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-124 amino acids from the Central region of human VDAC1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VDAC1
Additional Names:
Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC
Protein Accession Number:
P21796
Protein Gi Number:
130683
Ncbi Gene Id Number:
7416
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
Background Reference 1:
Shoshan-Barmatz,V., Biochim. Biophys. Acta 1787 (5), 421-430 (2009)
Background Reference 2:
Hiller,S., Science 321 (5893), 1206-1210 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-367

List Size:
400 ul

List Price:

$495.00

Datasheet
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