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Western blot analysis of lysates from bEnd.3 cells using VE-Cadherin Rabbit mAb (A25003) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 90s.
Western blot analysis of lysates from Rat lung using VE-Cadherin Rabbit mAb (A25003) at 1:1200 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using VE-Cadherin Rabbit mAb (A25003) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using VE-Cadherin Rabbit mAb (A25003) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using VE-Cadherin Rabbit mAb (A25003) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Flow cytometry: 1X10^6 C2C12 cells (negative control,left) and bEnd.3 cells (right) were surface-stained with VE-Cadherin Rabbit mAb (A25003,2 μg/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 bEnd.3 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,5 μl/Test,left) or VE-Cadherin Rabbit mAb (A25003,2 μg/mL,right).
Western blot analysis of lysates from bEnd.3 cells using VE-Cadherin Rabbit mAb (A25003) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 90s.

VE-Cadherin Rabbit Recombinant Antibody

CATALOG NUMBER: 83-040

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB
Application Note:
WB,1:500 - 1:2000, IHC-P,1:500 - 1:3000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
bEnd.3,Rat lung
Predicted Molecular Weight:
88kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Cdh5
Additional Names:
7B4; Vec; VECD; Cd144; VEcad; VE-Cad; VE-Cadherin
Protein Accession Number:
P55284
Ncbi Gene Id Number:
12562
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the cadherin family of calcium-dependent glycoproteins that mediate cell adhesion and regulate many morphogenetic events during development. The encoded preproprotein is further processed to generate a mature protein. Mice lacking the encoded protein die in utero due to vascular insufficiency, caused by increased endothelial apoptosis. Multiple distinct genes of the cadherin family, including this gene, are found on chromosome 8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-040

List Size:
100 uL

List Price:

$540.00

Datasheet
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