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Mouse VEGF164, Tag Free (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Mouse VEGF164, Tag Free (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

VEGF 164 Recombinant Protein (HEK293 Expressed)

CATALOG NUMBER: 96-771

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ala 27 - Arg 190
Fusion Tag:
Tag free
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
19.3 kDa
Biological Activity:
Immobilized Mouse VEGF164, Tag Free at 2ug/mL (100uL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL.
Purity:
>95% as determined by SDS-PAGE and SEC-HPLC.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Vegfa
Additional Names:
VEGF164, VEGFA, VPF
Protein Accession Number:
AAB22253.1
Ncbi Gene Id Number:
22339
Background:
Vascular endothelial growth factor A (VEGFA) is also known as Vascular permeability factor (VPF). VEGFA belongs to the PDGF/VEGF growth factor family. VEGFA is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. VEGFA is produced by a group of three major isoforms as a result of alternative splicing and if any three isoforms are produced (VEGFA120, VEGFA164, and VEGFA188) then this will not result in vessel defects and death of the full VEGFA knockout in mice.
Background Reference 1:
Shin S.-Y., et al., 2005, Yonsei Med. J. 46:679-686.
Background Reference 2:
Breier G., et al., 1992, Development 114:521-532.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-771

List Size:
0.02 mg

List Price:

$408.00

Datasheet
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