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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with VEGF antibody (VEGF/1063).
Formalin-fixed, paraffin-embedded human tonsil stained with VEGF antibody (VEGF/1063).
SDS-PAGE Analysis of Purified, BSA-Free VEGF Antibody (clone VEGF/1063). Confirmation of Integrity and Purity of the Antibody.
Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with VEGF antibody (VEGF/1063).

VEGF Antibody [VEGF/1063]

CATALOG NUMBER: 33-479

Clonality:
Monoclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Rabbit, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Dog, Human, Mouse, Rabbit, Rat
Immunogen:
Human recombinant protein was used as the immunogen for the VEGF antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)
Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.

Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
VEGFA
Additional Names:
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGF
Ncbi Gene Id Number:
7422
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-479

List Size:
100 ug

List Price:

$626.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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