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43-168 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-168 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-168 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-168 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VEGFA Antibody

CATALOG NUMBER: 43-168

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig
Immunogen:
The immunogen for this antibody is: C-RIKPHQGQHIGE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney and Lung) for this particular batch.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml.Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms.
Predicted Molecular Weight:
Approx 45kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VEGFA
Additional Names:
VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OTTHUMP00000224154, OTTHUMP00000224423, OTTHUMP00000224424, OTTHUMP00000224425, OTTHUMP00000224426, OTTHUMP00000224427, OTTHUMP00000224428, OTTHUMP00000224429, OTTHUMP00000224430, vascular permeability factor
Protein Accession Number:
NP_001020537.2,NP_003367.4,NP_001020538.2,NP_001020539.2,etc.
Protein Gi Number:
17380528
Ncbi Gene Id Number:
7422
Background Reference 1:
Weiss TW, Simak R, Kaun C, Rega G, Pflüger H, Maurer G, Huber K, Wojta J.Oncostatin M and IL-6 induce u-PA and VEGF in prostate cancer cells and correlate in vivo.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-168

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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