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IHC testing of FFPE human colon carcinoma with TL1A antibody (clone VEGI/1283). Required HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human parathyroid mass with TL1A antibody (clone VEGI/1283). Required HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free TL1A Antibody (clone VEGI/1283). Confirmation of Integrity and Purity of the Antibody.
IHC testing of FFPE human colon carcinoma with TL1A antibody (clone VEGI/1283). Required HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

VEGI / TL1A Antibody [VEGI/1283]

CATALOG NUMBER: 33-708

Clonality:
Monoclonal
Tested Applications:
Flow, IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
A full length human recombinant protein was used as the immunogen for this TL1A antibody.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE): 1-2 ug/ml
Titration of the TL1A antibody may be required for optimal performance.
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
TNFSF15
Additional Names:
TL1A Antibody: TL1, TL1A, VEGI, VEGI192A, TL1, Tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1
Ncbi Gene Id Number:
9966
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
TNF ligand-related molecule 1 is an anti-angiogenic cytokine that is also called Tumor necrosis factor superfamily, member 15 (TNFSF15) and Vascular endothelial growth inhibitor (VEGI/VEGI192A). This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Reduced expression of TL1A has been reported as a marker of poor prognosis in breast cancer.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-708

List Size:
100 ug

List Price:

$626.00

Datasheet
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