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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~53k VGAT protein.

Vesicular GABA transporter Antibody

CATALOG NUMBER: 50-194

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Dog, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Dog, Rat
Immunogen:
Peptide corresponding to amino acid residues from the N-terminal region of rat Vesicular GABA Amino Acid Transporter (VGAT).
Conjugate:
Unconjugated
Tested Applications:
WB
Predicted Molecular Weight:
53
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
Vesicular GABA transporter antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
Ncbi Official Symbol:
Slc32a1
Protein Accession Number:
O35458
Protein Gi Number:
29428082
Ncbi Gene Id Number:
83612
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).
Background Reference 1:
Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750.
Background Reference 2:
De GS, Schafer MK, Defamie N, Chen C, Ricci A, Weihe E, Varoqui H, Erickson JD (2005) Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 25:7121-7133.
Background Reference 3:
McIntire SL, Reimer RJ, Schuske K, Edwards RH, Jorgensen EM (1997) Identification and characterization of the vesicular GABA transporter. Nature (Lond ) 389:870-876.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

50-194

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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