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Western blot analysis of VEZT in 293 cell lysate with VEZT antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of VEZT in rat kidney tissue with VEZT antibody at 5 μg/ml.
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
Western blot analysis of VEZT in 293 cell lysate with VEZT antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

VEZT Antibody

CATALOG NUMBER: 7959

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT.
The immunogen is located within amino acids 480 - 530 of VEZT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 63, 68, 78, 81, 83, 86 kDa
Observed: 60 kDa
Purification:
VEZT antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VEZT antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VEZT antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
VEZT
Additional Names:
VEZT Antibody: VEZATIN, Vezatin
Protein Accession Number:
NP_060069
Protein Gi Number:
155030244
Ncbi Gene Id Number:
55591
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
Background Reference 1:
Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9.
Background Reference 2:
Guo X, Jing C, Zhang L, et al. Down-regulation of VEZT gene expression in human gastric cancer involves promoter methylation and miR-43c. Biochem. Biophys. Res. Commun. 2011; 404:622-7.
Background Reference 3:
Hyenne V, Louvet-Vallee S, El-Amraoui A, et al. Vezatin, a protein associated to adherins junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 2005; 287:180-91.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7959

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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