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Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of VGF in human brain with VGFantibody at 5 μg/mL.
Immunofluorescence of VGF in Human Brain cells with VGF antibody at 20 μg/mL.
Western blot analysis of VGF expression in human brain tissue lysate with VGF antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

VGF Antibody

CATALOG NUMBER: 4611

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
VGF antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human VGF.
The immunogen is located within the last 50 amino acids of VGF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
VGF antibody can be used for detection of VGF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 68 kDa
Observed: 70 kDa 
Purification:
VGF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VGF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VGF antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VGF
Additional Names:
VGF Antibody: Neurosecretory protein VGF, NERP-1
Protein Accession Number:
NP_003369
Protein Gi Number:
17136078
Ncbi Gene Id Number:
7425
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VGF Antibody: VGF was initially identified as a rapidly regulated gene product in nerve growth factor-treated PC12 cells. This protein is synthesized in neurons in the central and peripheral nervous system as well as in the pituitary, adrenal medulla, endocrine cells of the stomach, and pancreatic beta cells. VGF is thought to be involved in organism energy balance and regulation of homeostasis as mice lacking this gene show deficits in these areas. More recent results suggest that VGF is upregulated by brain-derived neurotrophic factor (BDNF) and can stimulate the proliferation of hippocampal progenitor cells and produce antidepressant-like behavioral effects, suggesting that this pathway may be a suitable target for therapeutic treatments.
Background Reference 1:
Levi A, Eldridge JD, and Paterson BM. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 1985; 229:393-5.
Background Reference 2:
Possenti R, Eldridge JD, Paterson BM, et al. A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulatory pathway. EMBO J.1989; 8:2217-23.
Background Reference 3:
Hahm S, Mizuno TM, Wu TJ, et al. Targeted deletion of the Vgf gene indicates that the encoded secretory peptide precursor plays a novel role in the regulation of energy balance. Neuron 1999; 23:537-48.
Background Reference 4:
Thakker-Varia S, Krol JJ, Nettleton J, et al. The neuropeptide VGF produces antidepressant-like behavioral effects and enhances proliferation in the hippocampus. J. Neurosci. 2007; 27:12156-67.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4611

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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