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43-649 (2ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-649 (0.1ug/ml) staining of Mouse (A) and (2ug/ml) Rat (B) Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed
43-649 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/Intermediate filament staining. The nuc
43-649 (2ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Vimentin Antibody

CATALOG NUMBER: 43-649

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-QVINETSQHHDDLE
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1). Recommended concentration: 0.1-2ug/ml.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended concentration: 5ug/ml.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Predicted Molecular Weight:
Approx 55kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Vimentin
Additional Names:
VIM, vimentin, FLJ36605
Protein Accession Number:
NP_003371.2
Protein Gi Number:
55977767
Ncbi Gene Id Number:
7431
Background Reference 1:
Maraina CH, Nurdayana AK, Rusni D, Azwany Y.Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-649

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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