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Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from A549 cells treated with Nocodazole using Phospho-Vimentin (Ser56) Antibody. The lane on the right is treated with the antigen-specific peptide.

Vimentin (phospho Ser56) Antibody

CATALOG NUMBER: 79-990

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Vimentin (phospho Ser56) antibody was raised against a peptide sequence around phosphorylation site of serine 56 (A-S-S (p)-P-G) derived from Human Vimentin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.
Predicted Molecular Weight:
54 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
VIM
Additional Names:
VIME, vimentin
Protein Accession Number:
NP_003371.2
Protein Gi Number:
62414289
Ncbi Gene Id Number:
7431
Background:
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
Background Reference 1:
Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-990

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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