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Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.
Western blot analysis of Vinculin in 293, A431, A549, Daudi, HeLa, HepG2, K562, 3T3, Raji, U937 cell lysate and mouse brain, rat brain, rabbit brain, mouse lung, rat lung, mouse liver, rat liver, rabbit spleen, chicken liver and chicken small intestine tissue lysate with Biotin-Vinculin antibody at 1 μg/ml.

Vinculin Antibody (biotin)

CATALOG NUMBER: 7807-biotin

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Chicken, Human, Mouse, Rabbit, Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Chicken, Human, Mouse, Rabbit, Rat
Immunogen:
Biotin-Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Specificity:
Biotin-Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 117 kDa
Observed: 117 kDa
Purification:
Biotin-Vinculin antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Biotin-Vinculin Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Biotin-Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
VCL
Additional Names:
CMD1W, CMH15, Metavinculin, MVCL
Protein Accession Number:
NP_003364
Protein Gi Number:
4507877
Ncbi Gene Id Number:
7414
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background Reference 1:
Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
Background Reference 2:
Gilmore et al. J. Cell Sci. 1992; 103:719-31.
Background Reference 3:
Deakin et al. PLoS One 2012; 7:e37990.
Background Reference 4:
Goldmann et al. Cell Biol. Int. 2013; Feb 1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7807-biotin

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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