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Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr821) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HeLa cells treated with Forskolin using Phospho-Vinculin (Tyr821) Antibody. The lane on the right is treated with the antigen-specific peptide.

Vinculin (phospho Tyr821) Antibody

CATALOG NUMBER: 80-022

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Vinculin (phospho Tyr821) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 821 (S-G-Y (p)-R-I) derived from Human Vinculin.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Vinculin only when phosphorylated at tyrosine 821.
Predicted Molecular Weight:
123 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
VCL
Additional Names:
Vinculin
Protein Accession Number:
NP_003364.1
Protein Gi Number:
4507877
Ncbi Gene Id Number:
7414
Background:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Background Reference 1:
Subauste MC, et al. (2004) J Cell Biol 165, 371-81
Background Reference 2:
Olsen JV, et al. (2010) Sci Signal 3, ra3
Background Reference 3:
Jørgensen C, et al. (2009) Science 326, 1502-9

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-022

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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