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Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Immunohistochemistry of VISA in human brain tissue with VISA antibody at 5 μg/mL.
Immunofluorescence of VISA in Human Brain cells with VISA antibody at 20 μg/mL.
Western blot analysis of VISA in A20 cell lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Trial Size

VISA Antibody

CATALOG NUMBER: 4053

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
VISA antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human VISA.
The immunogen is located within the first 50 amino acids of VISA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
VISA antibody can be used for detection of VISA by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
VISA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VISA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VISA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAVS
Additional Names:
VISA Antibody: IPS1, VISA, IPS-1, CARDIF, IPS1, KIAA1271, Mitochondrial antiviral-signaling protein, CARD adapter inducing interferon beta, MAVS
Protein Accession Number:
NP_065797
Protein Gi Number:
83776598
Ncbi Gene Id Number:
57506
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VISA Antibody: Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response. VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-b signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-κB. VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKα, IKKβ and IKKε kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system. At least three isoforms of VISA are known to exist.
Background Reference 1:
Seth RB, Sun L, and Chen ZJ. Antiviral innate immunity pathways. Cell Res.2006; 16:141-7.
Background Reference 2:
Xu LG, Wang YY, Han KJ, et al. VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol. Cell2005; 19:727-40.
Background Reference 3:
Meylan E, Curran J, Hofman K, et al. Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature2005; 1167-72.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4053

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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