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Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071, RF16072, RF16073, and RF16074 abs at decreasing concentrations.
Western blot analysis of VISTA in over expressing HEK293 cells using RF16071 antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.
Immunocytochemistry of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 1 μg/ml.
Immunofluorescence of VISTA in over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml.
Immunofluorescence of VISTA in human spleen tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml.
Immunohistochemistry of VISTA in human lymphoma tissue using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 2 μg/ml.
Flow cytometry analysis of VISTA over expressing HEK293 cells using (A) RF16071, (B) RF16072, (C) RF16073, (D) RF16074, and (E) control mouse IgG antibody at 10 μg/ml. Blue: untransfected HEK293 cells. Yellow: VISTA over expressing HEK293 cells.
Titration curve analysis of VISTA mAbs to detect recombinant LVISTA in ELISA with RF16071, RF16072, RF16073, and RF16074 abs at decreasing concentrations.

VISTA Detection Set (Risk Free)

CATALOG NUMBER: RF16070

Clonality:
Monoclonal
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
VISTA antibodies were raised against the extracellular domain of human VISTA.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, IF, IHC-P, WB
Set Contents:
VISTA Antibody [4C4], Cat. No. RF16071 (20 μg)

VISTA Antibody [6D2], Cat. No. RF16072 (20 μg)
VISTA Antibody [8E11], Cat. No. RF16073 (20 μg)
VISTA Antibody [9E4], Cat. No. RF16074 (20 μg)
Positive Control 1:
Cat. No. RF10001-03 - Human Spleen Tissue Lysate
Purification:
Antibodies are supplied as protein A purified IgG1 and IgG2b.
Clonality:
Monoclonal
Isotype:
IgG1, IgG2b
physical-state:
Liquid
Buffer:
PBS containing 0.02% sodium azide.
Concentration:
Antibody 1 mg/mL
Storage Conditions:
Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications
Background Reference 1:
Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
Background Reference 2:
Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.
Set Contents:
VISTA Antibody [4C4], Cat. No. RF16071 (20 μg)

VISTA Antibody [6D2], Cat. No. RF16072 (20 μg)
VISTA Antibody [8E11], Cat. No. RF16073 (20 μg)
VISTA Antibody [9E4], Cat. No. RF16074 (20 μg)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

RF16070

List Size:
1 Set

List Price:

$339.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

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