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Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Antibody staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Antibody staining VLDLR in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
VLDLR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

VLDLR Antibody

CATALOG NUMBER: 62-037

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This VLDLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-510 amino acids from the Central region of human VLDLR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VLDLR
Additional Names:
Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
Protein Accession Number:
P98155
Protein Gi Number:
1730111
Ncbi Gene Id Number:
7436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The low density lipoprotein receptor (LDLR) gene familyconsists of cell surface proteins involved in receptor-mediatedendocytosis of specific ligands. This gene encodes a lipoproteinreceptor that is a member of the LDLR family and plays importantroles in VLDL-triglyceride metabolism and the reelin signalingpathway. Mutations in this gene cause VLDLR-associated cerebellarhypoplasia. Alternative splicing generates multiple transcriptvariants encoding distinct isoforms for this gene. [provided byRefSeq].
Background Reference 1:
Sakai, K., et al. Brain Res. 1276, 11-21 (2009)
Background Reference 2:
Francis, P.J., et al. J. Med. Genet. 46(5):300-307(2009)
Background Reference 3:
Ananyeva, N.M., et al. Blood Coagul. Fibrinolysis 19(6):543-555(2008)
Background Reference 4:
Turkmen, S., et al. Eur. J. Hum. Genet. 16(9):1070-1074(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-037

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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