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Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.
Immunohistochemistry of VLK in rat lung tissue with VLK antibody at 2.5 μg/mL.
Immunofluorescence of VLK in rat lung tissue with VLK antibody at 5 μg/mL.
Western blot analysis of VLK expression in Jurkat cell lysate with VLK antibody at 0.5 μg/ml.
Western blot analysis of VLK in human lung tissue lysate with VLK antibody at 1 μg/mL.
Trial Size

VLK Antibody

CATALOG NUMBER: 5561

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
VLK antibody was raised against a 15 amino acid synthetic peptide from near the center of human VLK.
The immunogen is located within amino acids 320 - 370 of VLK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
VLK antibody can be used for detection of VLK by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 32, 54 kDa
Observed: 31, 55 kDa 
Purification:
VLK Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VLK Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VLK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Pkdcc
Additional Names:
VLK Antibody: Vlk, MAd1, Adtk1, ESTM17, Sgk493, X83346, AI115348, AW548124, Vlk, Protein kinase domain-containing protein, cytoplasmic, Protein kinase-like protein SgK493
Protein Accession Number:
NP_612379
Protein Gi Number:
238636897
Ncbi Gene Id Number:
91461
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VLK Antibody: VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm. It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups. VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme. It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi. Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality. It has been suggested that mutations in VLK may be associated with the allergic condition atopy.
Background Reference 1:
Kinoshita M, Era T, Jakt LM, et al. The novel protein kinase Vlk is essential for stromal function of mesenchymal cells. Development2009; 136:2069-79.
Background Reference 2:
Castro-Giner F, Bustamante M, Ramon Gonzalez J, et al. A pooling-based genome-wide analysis identifies new potential candidate genes for atopy in the European Community Respiratory Health Survey (ECRHS). BMC Med. Genet.2009; 10:128.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5561

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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