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43-568 (0.1ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-568 (0.1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-568 (0.1ug/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS16, Antibody

CATALOG NUMBER: 43-568

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KESQKADEYLREIQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7kDa according to NP_072097.2). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognize reported isoform 1 (NP_072097.2) only.
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS16
Additional Names:
VPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), hVPS16, OTTHUMP00000030047, OTTHUMP00000030048, vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog
Protein Accession Number:
NP_072097.2
Protein Gi Number:
17978479
Ncbi Gene Id Number:
64601
Background Reference 1:
Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H.Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-568

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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