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43-569 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-569 (0.5ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-569 (1ug/ml) staining of HepG2 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS16 Antibody

CATALOG NUMBER: 43-569

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: CYKVQQKDVSDED
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 95+80kDa band observed in lysates of cell line HepG2 and 95kDa in Mouse Spleen (calculated MW of 94.7kDa according to NP_072097.2 and 78.3kDa according to NP_536338.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_072097.2; NP_536338.1).
Predicted Molecular Weight:
Approx 95 + 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS16
Additional Names:
VPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), hVPS16, OTTHUMP00000030047, OTTHUMP00000030048, vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog
Protein Accession Number:
NP_072097.2,NP_536338.1
Protein Gi Number:
17978479
Ncbi Gene Id Number:
64601
Background Reference 1:
Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H.Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-569

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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