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Western blot analysis in kidney cell line lysates (35ug/lane).
Western blot analysis in kidney cell line lysates (35ug/lane).

VPS25 Antibody

CATALOG NUMBER: 60-656

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
21 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS25
Additional Names:
Vacuolar protein-sorting-associated protein 25, hVps25, Dermal papilla-derived protein 9, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, VPS25, DERP9, EAP20
Protein Accession Number:
Q9BRG1
Protein Gi Number:
73920459
Ncbi Gene Id Number:
84313
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) formESCRT-II (endosomal sorting complex required for transport II), acomplex involved in endocytosis of ubiquitinated membrane proteins.VPS25, VPS36, and SNF8 are also associated in a multiproteincomplex with RNA polymerase II elongation factor (ELL; MIM 600284)(Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001[PubMed 11278625]).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Background Reference 3:
Im, Y.J., et al. Dev. Cell 17(2):234-243(2009)
Background Reference 4:
Lamesch, P., et al. Genomics 89(3):307-315(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-656

List Size:
400 ul

List Price:

$495.00

Datasheet
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