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46-579 staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
HEK293 overexpressing VPS26A and probed with 46-579 (mock transfection in first lane).
46-579 staining (1ug/ml) of Human Kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

VPS26A Antibody

CATALOG NUMBER: 46-579

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SPESQASAEQPEM
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 40kDa band observed in Human Kidney lysates (calculated MW of 38.2 kDa according to NP_004887.2). In transfected HEK293 transiently expressing VPS26A a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_004887.2) only.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS26A
Additional Names:
VPS26A, vacuolar protein sorting 26 homologue A (yeast), FLJ12930, HB58, Hbeta58, VPS26, Hbeta58 homolog, vacuolar protein sorting 26 (yeast homolog), vacuolar protein sorting 26 (yeast), vacuolar protein sorting 26 homolog A, vacuolar protein sorting 26 homolog A (S. pombe), PEP8A, OTTHUMP00000019721, vacuolar protein sorting 26 A
Protein Accession Number:
NP_004887.2
Protein Gi Number:
17978519
Ncbi Gene Id Number:
9559
Background Reference 1:
Haft CR, de la Luz Sierra M, Bafford R, Lesniak MA, Barr VA, Taylor SI. Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes. Mol Biol Cell. 2000 Dec;11(12):4105-16.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-579

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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