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Western blot analysis of VPS26A Antibody in HepG2 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VPS26A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of VPS26A Antibody in HepG2 cell line lysates (35ug/lane)

VPS26A Antibody

CATALOG NUMBER: 63-613

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse, Rat, C. Elegans
Immunogen:
This VPS26A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the Central region of human VPS26A.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
38 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS26A
Additional Names:
Vacuolar protein sorting-associated protein 26A, Vesicle protein sorting 26A, hVPS26, VPS26A, VPS26
Protein Accession Number:
O75436
Ncbi Gene Id Number:
9559
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VPS26A is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Background Reference 1:
Haft,C.R., et.al., Mol. Biol. Cell 11 (12), 4105-4116 (2000)
Background Reference 2:
Reddy,J.V.et.al., Mol. Biol. Cell 12 (10), 3242-3256 (2001)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-613

List Size:
400 ul

List Price:

$495.00

Datasheet
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