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46-585 (0.03g/ml) staining of human duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-585 (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-585 (0.03g/ml) staining of human duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VPS37C Antibody

CATALOG NUMBER: 46-585

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ETLKDKTLQELEELQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in human duodenum lysates (calculated MW of 38.7kDa according to NP_060436.4). Recommended concentration: 0.03 - 0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows strong cytoplasm staining in stromal cells. Recommended concentration, 3-6ug/ml.
Positive Control 1:
Cat. No. 1364 - Human Duodenum Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS37C
Additional Names:
VPS37C, vacuolar protein sorting 37C (yeast) , FLJ20847 , vacuolar protein sorting 37C, vacuolar protein sorting 37 homolog C (S. cerevisiae)
Protein Accession Number:
NP_060436.4
Protein Gi Number:
57863314
Ncbi Gene Id Number:
55048
Background Reference 1:
Eastman SW, Martin-Serrano J, Chung W, Zang T, Bieniasz PD. Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding. J Biol Chem. 2005 Jan 7;280(1):628-36. Epub 2004 Oct 27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-585

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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