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Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of VPS39 in mouse liver tissue with VPS39 antibody at 5 μg/mL.
Immunofluorescence of VPS39 in mouse liver tissue with VPS39 antibody at 20 μg/mL.
Immunofluorescence of VPS39 in human liver tissue with VPS39 antibody at 5 μg/ml. <br><br>Green: VPS39 antibody (6183) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of VPS39 in rat liver tissue lysate with VPS39 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

VPS39 Antibody

CATALOG NUMBER: 6183

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
VPS39 antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of human VPS39.
The immunogen is located within amino acids 710 - 760 of VPS39.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
VPS39 antibody can be used for detection of VPS39 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of VPS39 are known to exist; this antibody will detect all four. VPS39 antibody is predicted not to cross-react with TRAP1.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
VPS39 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VPS39 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VPS39 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS39
Additional Names:
VPS39 Antibody: TLP, VAM6, hVam6p, KIAA0770, TLP, TRAP1-like protein
Protein Accession Number:
AAQ05978
Protein Gi Number:
54234024
Ncbi Gene Id Number:
23339
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VPS39 Antibody: VPS39 is the mammalian homolog of the yeast vacuolar protein VAM6, that in mammals, promotes lysosome clustering and fusion in vivo. VPS39 is also thought to modulate the TGF-beta response by coupling the TGF-beta receptor complex to the Smad pathway. Similar to the related protein TRAP1, the VPS39 protein is essential for early embryonic development, with mice lacking VPS39 dying at or before E6.5.
Background Reference 1:
Nakamura N, Hirata A, Ohsumi Y, et al. Vam2/Vps41p and Vam6/Vps39p are components of a protein complex on the vacuolar membranes and involved in the vacuolar assembly in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1997; 272:11344-9.
Background Reference 2:
Caplan S, Hartnell LM, Aguilar RC, et al. Human Vam6p promotes lysosome clustering and fusion in vivo. J. Cell Biol. 2001; 154:109-22.
Background Reference 3:
Felici A, Wurthner JU, Parks WT, et al. TLP, a novel modulator of TGF-beta signaling, has opposite effects on Smad2- and Smad3-dependent signaling. EMBO J. 2003; 22:4465-77.
Background Reference 4:
Messler S, Kropp S, Episkopou V, et al. The TGF-b signaling modulators TRAP1/TGFBRAP1 and VPS39/Vam6/TLP are essential for early embryonic development. Immunobiology 2011; 216:343-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6183

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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