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46-586 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
46-586 (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-586 (3.75ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-586 (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

VPS41 Antibody

CATALOG NUMBER: 46-586

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DHLKKDSQNKTLLK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration: 4-6ug/ml. Paraffin embedded Human Placenta and Prostate. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS41
Additional Names:
VPS41, vacuolar protein sorting 41 (yeast) , HGNC:12713, HVSP41, vacuolar assembly protein 41, vacuolar protein sorting 41 (yeast homolog), vacuolar protein sorting 41 homolog (S. cerevisiae), HVPS41, hVps41p, vacuolar protein sorting 41
Protein Accession Number:
NP_055211.2,NP_542198.2
Protein Gi Number:
7657677
Ncbi Gene Id Number:
27072
Background Reference 1:
McVey Ward D, Radisky D, Scullion MA, Tuttle MS, Vaughn M, Kaplan J. hVPS41 is expressed in multiple isoforms and can associate with vesicles through a RING-H2 finger motif. Exp Cell Res. 2001 Jul 1;267(1):126-34.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-586

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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