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46-587 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-587 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-587 (5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-587 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-587 (0.3ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VPS45 Antibody

CATALOG NUMBER: 46-587

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ESSQVTSRSASRR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex), Placenta and Small Intestine. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VPS45
Additional Names:
VPS45, VPS45A, VPS45B, VSP45, VSP45A, H1, H1VPS45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A (yeast), vacuolar protein sorting 45B (yeast), vacuolar protein sorting protein similar to S. cerevisiae Vsp45p, RP11-458I7.2, VPS54A
Protein Accession Number:
NP_009190.2
Protein Gi Number:
18105063
Ncbi Gene Id Number:
11311
Background Reference 1:
Rajasekariah P, Eyre HJ, Stanley KK, Walls RS, Sutherland GR. Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45). Int J Biochem Cell Biol. 1999 Jun;31(6):683-94.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-587

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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