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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
VPS52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of VPS52 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

VPS52 Antibody

CATALOG NUMBER: 55-511

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This VPS52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 610-637 amino acids from the C-terminal region of human VPS52.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS52
Additional Names:
Vacuolar protein sorting-associated protein 52 homolog, SAC2 suppressor of actin mutations 2-like protein, VPS52, SACM2L
Protein Accession Number:
Q8N1B4
Protein Gi Number:
74750887
Ncbi Gene Id Number:
6293
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
Background Reference 1:
Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009)Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009) Perez-Victoria, F.J., et al. Mol. Biol. Cell 19(6):2350-2362(2008) Liewen, H., et al. Exp. Cell Res. 306(1):24-34(2005) Mungall, A.J., et al. Nature 425(6960):805-811(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-511

List Size:
400 ul

List Price:

$495.00

Datasheet
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