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Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of VPS53 in 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

VPS53 Antibody

CATALOG NUMBER: 4595

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (78%)
Immunogen:
VPS53 antibody was raised against a 18 amino acid synthetic peptide from near the center of human VPS53.
The immunogen is located within amino acids 390 - 440 of VPS53.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
VPS53 antibody can be used for detection of VPS53 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Purification:
VPS53 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VPS53 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VPS53 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS53
Additional Names:
VPS53 Antibody: HCCS1, hVps53L, pp13624, Vacuolar protein sorting-associated protein 53 homolog
Protein Accession Number:
EAW90658
Protein Gi Number:
119611064
Ncbi Gene Id Number:
55275
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VPS53 Antibody: The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.
Background Reference 1:
Pfeffer SR. Targeting of proteins to the lysosome. Curr. Top. Microbiol. Immunol.1991; 170:43-65.
Background Reference 2:
Liewen H, Meinhold-Heerlein I, Oliveira V, et al. Characterization of the human GARP (Golgi associated retrograde protein) complex. Exp. Cell Res.2005; 306:22-34.
Background Reference 3:
Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell2008; 19:2350-62.
Background Reference 4:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4595

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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