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Western blot analysis in mouse lung tissue lysates (35ug/lane).
VPS54 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse lung tissue lysates (35ug/lane).

VPS54 Antibody

CATALOG NUMBER: 56-744

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This VPS54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 465-491 amino acids from the Central region of human VPS54.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
111 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VPS54
Additional Names:
Vacuolar protein sorting-associated protein 54, Hepatocellular carcinoma protein 8, Tumor antigen HOM-HCC-8, Tumor antigen SLP-8p, VPS54, HCC8
Protein Accession Number:
Q9P1Q0
Protein Gi Number:
82583721
Ncbi Gene Id Number:
51542
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes for a protein that in yeast forms partof a trimeric vacuolar-protein-sorting complex that is required forretrograde transport of proteins from prevacuoles to the late Golgicompartment. As in yeast, mammalian Vps54 proteins contain acoiled-coil region and dileucine motifs. Alternative splicingresults in multiple transcript variants encoding different isoforms
Background Reference 1:
Perez-Victoria, F.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12860-12865(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Perez-Victoria, F.J., et al. Mol. Cell. Biol. 29(19):5251-5263(2009)
Background Reference 4:
Meisler, M.H., et al. Amyotroph Lateral Scler 9(3):141-148(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-744

List Size:
400 ul

List Price:

$495.00

Datasheet
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