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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: 293T/17 whole cell lysates Lane 3: MCF-7 whole cell lysates Lysates/proteins at 20 ug per lane.

VRK1 Antibody

CATALOG NUMBER: 62-888

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This VRK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-396 amino acids from the Central region of human VRK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VRK1
Additional Names:
Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1
Protein Accession Number:
Q99986
Protein Gi Number:
45593726
Ncbi Gene Id Number:
7443
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Background Reference 1:
Nezu, J., et al., Genomics 45(2):327-331 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-888

List Size:
400 ul

List Price:

$495.00

Datasheet
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