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43-551 (2ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-551 (2ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

VRK2 Antibody

CATALOG NUMBER: 43-551

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-PNSQKVDSQKAATK
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in nuclear lysates of cell line K562 (calculated MW of 58.1kDa according to NP_001123954.1). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognize all isoforms (NP_001123952.1, NP_001123954.1, NP_001123955.1, NP_001129499.1). Reported variants represent identical protein: NP_001123953.1, NP_006287.2.
Predicted Molecular Weight:
Approx 60kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VRK2
Additional Names:
VRK2, vaccinia related kinase 2, vaccinia virus B1R-related kinase 2, vaccinia-related kinase-2
Protein Accession Number:
NP_001129499.1,NP_006287.2,NP_001123954.1,NP_001123955.1
Protein Gi Number:
209862855
Ncbi Gene Id Number:
7444
Background Reference 1:
Sanz-García M, López-Sánchez I, Lazo PA.Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-551

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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