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Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected the VSGP/F-spondin protein (arrow).
VSGP/F-spondin Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the detected the VSGP/F-spondin protein (arrow).

VSGP/F-spondin Antibody

CATALOG NUMBER: 56-410

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This VSGP/F-spondin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human VSGP/F-spondin.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
91 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPON1
Additional Names:
Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor, SPON1, KIAA0762, VSGP
Protein Accession Number:
Q9HCB6
Protein Gi Number:
52783472
Ncbi Gene Id Number:
10418
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
Background Reference 1:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 2:
Attur, M.G., et al. FASEB J. 23(1):79-89(2009)
Background Reference 3:
Tan, K., et al. J. Mol. Biol. 381(5):1213-1223(2008)
Background Reference 4:
Kitagawa, M., et al. Biochem. Biophys. Res. Commun. 349(3):1050-1056(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-410

List Size:
400 ul

List Price:

$495.00

Datasheet
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