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46-589 (1ug/ml) staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) and (0.1ug/ml) Human pancreas iso 1 (E) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-589 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cell junction and cytoplasmic staining. T
46-589 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
46-589 (1ug/ml) staining of HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B). Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D) and (0.1ug/ml) Human pancreas iso 1 (E) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

VTCN1 Antibody

CATALOG NUMBER: 46-589

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-SKGKGNANLEYK
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calculated MW of 30.9kDa according to NP_078902.2 isoform 1 and 20.7kDa according to NP_001240778.1 isoform 2 ). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
VTCN1
Additional Names:
B7-H4, B7H4, B7S1, B7X, B7h.5, FLJ22418, PRO1291, RP11-229A19.4, VCTN1, T cell costimulatory molecule B7x, immune costimulatory protein, V-set domain containing T cell activation inhibitor 1
Protein Accession Number:
NP_078902.2,NP_001240778.1
Protein Gi Number:
99028881
Ncbi Gene Id Number:
79679
Background Reference 1:
Krambeck AE, Thompson RH, Dong H, Lohse CM, Park ES, Kuntz SM, Leibovich BC, Blute ML, Cheville JC, Kwon ED. B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival. Proc Natl Acad Sci USA. 2006 Jul 5;103(27):10391-6. Epub 2006 Jun 23.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-589

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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