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Western blot analysis of VTI1a in human brain tissue lysate with VTI1a antibody at 1 μg/ml.
Immunohistochemistry of VTI1a in human brain tissue with VTI1a antibody at 5 μg/mL.
Western blot analysis of VTI1a in human brain tissue lysate with VTI1a antibody at 1 μg/ml.
Trial Size

VTI1a Antibody

CATALOG NUMBER: 8287

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
VTI1a antibody was raised against a 19 amino acid peptide near the center of human VTI1a.
The immunogen is located within amino acids 50 - 100 of VTI1a.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
VTI1a antibody can be used for detection of VTI1a by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
VTI1a antibody is human, mouse and rat reactive. At least two isoforms of VTI1a are known to exist; this antibody will detect both isoforms. VTI1a antibody is predicted to not cross-react with VTI1b.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 24, 27 kDa
Observed: 26 kDa
Purification:
VTI1a antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
VTI1a antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
VTI1a antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
VTI1a
Additional Names:
Vesicle transport through interaction with t-SNAREs 1A, MMDS3, MVti1, VTI1RP2, Vti1-rp2
Protein Accession Number:
NP_660207
Protein Gi Number:
113374156
Ncbi Gene Id Number:
143187
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). VTI1a mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane and promotes fusion of the lipid bilayers (4). VTI1a may be concerned with increased secretion of cytokines associated with cellular senescence (5).
Background Reference 1:
Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30.
Background Reference 2:
Kreykenbohm V, Wenzel D, Antonin W et al. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. Eur. J. Cell Biol. 2002; 81:273-80.
Background Reference 3:
Antonin W, Riedel D, von Mollard GF, et al. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. J. Neurosci. 2000; 20:5724-32.
Background Reference 4:
Atlashkin V, Kreykenbohm V, Eskelinen EL, et al. Deletion of the SNARE vti1b in mice results in the loss of a single SNARE partner, syntaxin 8. Mol. Cell Biol. 2003; 23:5198-207.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8287

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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