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Western blot analysis of VTN Antibody in NCI-H460(lane 1), HepG2(lane 2) cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with VTN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of NCI-H292 cells using VTN Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of VTN Antibody in NCI-H460(lane 1), HepG2(lane 2) cell line lysates (35ug/lane)

VTN Antibody

CATALOG NUMBER: 62-933

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This VTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-379 amino acids from the C-terminal region of human VTN.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
VTN
Additional Names:
Vitronectin, VN, S-protein, Serum-spreading factor, V75, Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B, VTN
Protein Accession Number:
P04004
Protein Gi Number:
139653
Ncbi Gene Id Number:
7448
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.
Background Reference 1:
Jenne D.E., Stanley K.K.EMBO J. 4:3153-3157(1985)
Background Reference 2:
Sigurdardottir O., Wiman B.Biochim. Acta 1208:104-110(1994)
Background Reference 3:
Seiffert D., Loskutoff D.J.J. Biol. Chem. 266:2824-2830(1991)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-933

List Size:
400 ul

List Price:

$495.00

Datasheet
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