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Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 μg/mL.
Immunohistochemistry of WAC in mouse testis tissue with WAC antibody at 2.5 μg/ml.
Immunofluorescence of WAC in mouse testis tissue with WAC antibody at 5 μg/ml.
Western blot analysis of WAC in human testis tissue lysate with WAC antibody at 1 μg/mL.
Trial Size

WAC Antibody

CATALOG NUMBER: 7293

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
WAC antibody was raised against a 19 amino acid peptide near the center of human WAC .
The immunogen is located within amino acids 210 - 260 of WAC.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WAC Antibody can be used for detection of WAC by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of WAC are known to exist; this antibody will detect the larger isoform.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 71 kDa
Observed: 75 kDa
Purification:
WAC Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WAC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WAC antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
WAC
Additional Names:
WAC Antibody: Wwp4, BM-016, PRO1741, KIAA1844, WW domain-containing adapter protein with coiled-coil
Protein Accession Number:
NP_057712
Protein Gi Number:
18379328
Ncbi Gene Id Number:
51322
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WAC Antibody: The WW domain containing adaptor with coiled-coil protein (WAC) contains a WW domain that mediates protein-protein interactions and colocalizes with RNA splicing factor SC35. Further studies have indicated that WAC is a functional partner of the RNF20/40 complex that ubiquitinates Histone H2B, and that WAC regulates H2B ubiquitination. WAC targets RNF20/40 to associate with RNA polymerase II complex for H2B ubiquitination at active transcription sites. WAC-dependent transcription is also important for cell-cycle checkpoint activation in response to genotoxic stress.
Background Reference 1:
Xu GM and Arnaout MA. WAC, a novel WW domain-containing adapter with a coiled-coil region, is colocalized with splicing factor SC35. Genomics 2002; 79:87-94.
Background Reference 2:
Zhang F and Yu X. WAC, a functional partner of RNF20/40, regulates histone H2B ubiquitination and gene transcription. Mol. Cell 2011; 41:384-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7293

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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