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Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of WAPL in rat heart tissue with WAPL antibody at 5 μg/mL.
Immunofluorescence of WAPL in rat heart tissue with WAPL antibody at 20 μg/mL.
Western blot analysis of WAPL in A20 cell lysate with WAPL antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

WAPL Antibody

CATALOG NUMBER: 7799

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
WAPL antibody was raised against an 18 amino acid peptide near the amino terminus of human WAPL.
The immunogen is located within amino acids 30 - 80 of WAPL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WAPL antibody can be used for detection of WAPL by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 131 kDa
Observed: 140 kDa
Purification:
WAPL antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WAPL antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WAPL antibody antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WAPAL
Additional Names:
WAPL Antibody: FOE, WAPL, KIAA0261, FOE, Wings apart-like protein homolog, Friend of EBNA2 protein
Protein Accession Number:
NP_055860
Protein Gi Number:
42734325
Ncbi Gene Id Number:
23063
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WAPL (wings apart-like), also known as WAPAL or FOE, is a 1,190 amino acid protein that contains one WAPL domain and may play an important role in cell growth (1). It is expressed in an isoform dependent manner in heart, skeletal muscle (isoform 2) and uterine cervix tumor tissue (isoform 1) (1,2,4). WAPL is involved in sister-chromatid adhesion and promotes release of cohesin from chromosomes by directly interacting with its regulatory subunits (3,4). WAPL is a new regulator of the development and metastasis of cancerous tissue (4,5).
Background Reference 1:
Kwiatkowski BA, Ragoczy T, Ehly J, et al. Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2. Exp. Cell Res. 2004; 300:223-33.
Background Reference 2:
Kueng S, Hegemann B, Peters BH, et al. Wapl controls the dynamic association of cohesion with chromatin. Cell 2006; 127:955-67.
Background Reference 3:
Gandhi R, Gillespie PJ, and Hirano T. Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Curr. Biol. 2006; 16:2406-17.
Background Reference 4:
Oikawa K, Ohbayashi T, Kiyono T, et al. Expression of a novel human gene, human wings apart-like (hWAPL), is associated with cervical carcinogenesis and tumor progression. Cancer Res. 2004; 64:3545-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7799

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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